Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification
نویسندگان
چکیده
منابع مشابه
Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملبررسی کارایی روشLAMP (Loop-mediated isothermal DNA amplification)
مقدمه:سالمونلاها از مهمترین باکتری های بیماری و عامل تیفوئید، باکتریمی، آنتروکولیت و سالمونلوز است که از عفونت های مشترک انسان و حیوانات می باشد. بیماریهای ناشی از این عامل یک معضل بزرگ بهداشتی در جهان به خصوص در کشورهای در حال توسعه از جمله کشور ما می باشد. بنابراین تشخیص سریع، دقیق و به موقع آن برای جلوگیری از اپیدمی و عوارض مخرب این باکتری ضروری به نظر می رسد. برای تشخیص سالمونلا روش های مخت...
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Ralstonia solanacearum is a pathogenic bacterium that causes wilt in over 200 plant species. Here we report a rapid and sensitive detection of R. solanacearum using an isothermal method for copying DNA known as loop-mediated amplification (LAMP). A set of four primers was designed to replicate the gene coding for the flagellar subunit, fliC, and conditions for detection were optimized to comple...
متن کاملDetection of Shigella in Milk and Clinical Samples by Magnetic Immunocaptured-Loop-Mediated Isothermal Amplification Assay
Shigella is an important human food-borne zoonosis bacterial pathogen, and can cause clinically severe diarrhea. There is an urgent need to develop a specific, sensitive, and rapid methodology for detection of this pathogen. In this study, loop-mediated isothermal amplification (LAMP) combined with magnetic immunocapture assay (IC-LAMP) was first developed for the detection of Shigella in pure ...
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ژورنال
عنوان ژورنال: BioScience Trends
سال: 2014
ISSN: 1881-7815,1881-7823
DOI: 10.5582/bst.2014.01118